EasyPrep Cell&Bacteria RNAprep Purification Kit
EasyPrep Cell&Bacteria RNAprep Purification Kit provides a fast, simple, and cost-effective method for purification of total RNA from cultured animal cells and bacteria. The purified RNA is ready for use in downstream applications such as: RT-PCR and real-time RT-PCR, gene-chips assay, northern blot, dot blot, polyA screening, in vitro transcript, and molecular cloning.
Features
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Column binding system.
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No phenol / chloroform extraction.
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Complete removal of genomic DNA contamination by DNase I.
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High yield, up to 35ug RNA from 1x10^6 cells.
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Purify RNA in 1 hour
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Work Flow
Yield of total RNA with EasyPrep Cell&Bacteria RNAprep Purification Kit:
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Source
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Yield of total RNA (1 x 106)
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Maximum number of cells
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COS
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Up to 35 µg
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3 x 106
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Hela
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Up to 15 µg
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7 x 106
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NH/3T3
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Up to 10 µg
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10 x 106
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RNA QC data:
Total RNA was purified from human Jurkat cells
using EasyPrep Cell&Bacteria RNAprep Purification
Kit. Elution volume: 50ul; Loading: 3ul/lane
